Anti-Kinesin-like protein KIF7

Application

Research CategoryCell Structure

This rabbit polyclonal Anti-Kinesin-like protein KIF7 Antibody, Cat. No. ABS1485 is validated for use in Western Blotting for the detection of Kinesin-like protein KIF7.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Kinesin-like protein KIF7 (UniProt: Q2M1P5; also known as KIF7) is encoded by the KIF7 (also known as UNQ340/PRO539) gene (Gene ID: 374654) in human. KIF7 is a member of kinesin-4 family that is essential for hedgehog signaling regulation. It localizes to cilia tips, the site of microtubule plus-ends, where it limits cilia length and controls cilia structure. KIF7 is required for the normal structure of primary cilia and in the absence of KIF7, cilia are long and unstable. KIF7 acts as both a negative and positive regulator of sonic hedgehog (Shh) and Indian hedgehog (Ihh) pathways, acting downstream of SMO, through both SUFU-dependent and -independent mechanisms. It negatively regulates Shh signaling by preventing inappropriate activation of the transcriptional activator Gli2 in the absence of ligand and positively regulates Shh signaling by preventing the processing of the Gli3 into its repressor form. KIF7 physically interacts with Gli transcription factors and controls their proteolysis and stability. Defects in KIF7 gene can lead to ciliary dysfunction, which result in a broad spectrum of disorders, collectively termed ciliopathies, including the Bardet-Biedl syndrome, a syndrome characterized by severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation, and mental retardation. (Ref.: Cheung. H.O. et al. (2009). Sci. Signal. 2(76):ra29).

Immunogen

KLH-cojugated linear peptide corresponding to 14 amino acid fragment from the C-terminal region of human Kinesin-like protein KIF7.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected Kinesin-like protein KIF7 in 10 µg of HEK293 cell lysate.

Specificity

Detects Kinesin-like protein KIF7 in HEK293 cells by Western blotting.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~160 kDa observed; 150.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).